Figure 5.

In vivo tracheobronchial biopsy from patient with CAPA and invasive Aspergillus tracheobronchitis

(A) Slide stained with Grocott–Gomori's methenamine silver, which makes fungi appear black and the background tissue green. (B) RNAScope image, in which the red puncta reflect SARS-CoV-2 nucleocapsid RNA. Epithelial cells are labelled green by anti-KRT8 antibody staining. DAPI was used as a nuclear stain (grey). (C) Slide stained with haematoxylin and eosin, which is used to look at damaged areas. (D–E) Two magnified areas of the CAPA biopsy, with respective haematoxylin and eosin-stained, Grocott-Gomori's methenamine silver-stained, and RNAScope images. Examples of fungi are indicated on the Grocott-Gomori's methenamine silver-stained images by arrows. The asterisks localise the lumen of the respiratory tract. CAPA=COVID-19-associated pulmonary aspergillosis.