TABLE 1.
Properties of the ORFs in the cps locus of S. agalactiae type Ia and homologies with gene products of other bacteria
| ORF | Nucleotide position in sequence | No. of amino acids | Proposed function of gene product | Similar gene product(s) (% identity) | Reference |
|---|---|---|---|---|---|
| cpsIaA | 151–1605 | 485 | Regulationb | Streptococcus agalactiae type III CpsXd (100%) | 28 |
| Streptococcus pneumoniae serotype 14 Cps14Ad (50.2%) | 27 | ||||
| Streptococcus pneumoniae serotype 19 Cps19fAd (50.6%) | 16 | ||||
| Bacillus subtilisLytRd (32.9%) | 29 | ||||
| cpsIaB | 1614–2342 | 243 | Unknown | Streptococcus agalactiae type III CpsAd (99.2%) | 38 |
| Streptococcus pneumoniae serotype 14 Cps14Bd (62.6%) | 26 | ||||
| Streptococcus pneumoniae serotype 19 Cps19fBd (64.2%) | 16 | ||||
| cpsIaC | 2354–3043 | 230 | Chain length regulatorb | Streptococcus agalactiae type III CpsBd (99.1%) | 38 |
| Streptococcus pneumoniae serotype 14 Cps14Cd (44.2%) | 26 | ||||
| Streptococcus pneumoniae serotype 19 Cps19fCd (45.6%) | 16 | ||||
| Rhizobium melilotiExoPde (22.5%) | 15 | ||||
| cpsIaD | 3057–3743 | 229 | Chain length regulator or exportb | Streptococcus agalactiaetype III CpsCd (99.1%) | 38 |
| Streptococcus pneumoniae serotype 14 Cps14Dd (55.9%) | 26 | ||||
| Streptococcus pneumoniae serotype 19 Cps19fDd (56.8%) | 16 | ||||
| Rhizobium melilotiExoPdf (29.6%) | 15 | ||||
| cpsIaE | 3973–5145 | 391 | Glucosyltransferasea | Streptococcus agalactiae type III CpsDd (98.9%) | 38 |
| Streptococcus pneumoniae serotype 14 Cps14Ec (49.1%) | 25 | ||||
| Streptococcus pneumoniae serotype 19 Cps19fEd (49.1%) | 16 | ||||
| Salmonella entericaRfbPd (32.1%) | 47 | ||||
| cpsIaF | 5172–5618 | 149 | Unknown | Streptococcus pneumoniae serotype 14 Cps14F (83.9%) | 26 |
| Sphingomonas S88 SpsKde (33.1%) | 50 | ||||
| cpsIaG | 5621–6160 | 180 | β-1,4-galactosyltransferasea | Streptococcus pneumoniae serotype 14 Cps14Gc (53.2%) | 26 |
| Sphingomonas S88 SpsKdf (23.6%) | 50 | ||||
| cpsIaH | 6202–7338 | 379 | Putative CP polymeraseb | Streptococcus pneumoniaeserotype 14 Cps14Hd (23.6%) | 26 |
| Salmonella typhimuriumRfcd (19.5%) | 9 | ||||
| Shigella flexneriRfcd (16.1%) | 32 | ||||
| cpsIaI | 7341–8339 | 333 | β-1,3-N-acetylglucosaminyltransferasea | Streptococcus thermophilus Sfi6 EpsId (34.2%) | 42 |
| Streptococcus pneumoniae serotype 14 Cps14Ic (26.4%) | 27 | ||||
| Rhizobium melilotiExoWd (25.5%) | 14 | ||||
| Rhizobium melilotiExoUd (23.7%) | 14 | ||||
| Neisseria meningitidisLgtAd (20.7%) | 21 | ||||
| cpsIaJ | 8347–9291 | 315 | β-1,4-galactosyltransferasea | Streptococcus pneumoniae serotype 14 Cps14Jc (37.0%) | 27 |
| Streptococcus thermophilus Sfi6 Eps Id (30.3%) | 42 | ||||
| Neisseria meningitidisLgtAd (26.5%) | 21 | ||||
| Rhizobium melilotiExoOd (26.5%) | 14 | ||||
| Rhizobium melilotiExoUd (26.7%) | 14 | ||||
| cpsIaK | 9378–10331 | 318 | Unknown | Haemophilus influenzae type b Orf4f (20.8%) | 44 |
| cpsIaL | 10331–11728 | 466 | Repeat unit transporterb | Shigella dysenteriaeRfbXd (19.8%) | 23 |
| Staphylococcus aureusCapFd (22.2%) | 30 | ||||
| Streptococcus pneumoniae serotype 14 Cps14Ld (16.4%) | 27 | ||||
| neuB | 11731–12753 | 341 | Sialic acid synthesisb | Escherichia coliNeuBd (56.0%) | 1 |
| Neisseria meningitidis group B NeuBd (35.7%) | 12 | ||||
| neuC | 12833–13984 | 384 | Sialic acid synthesisb | Escherichia coliNeuCd (43.6%) | 51 |
| Neisseria meningitidis group B NeuCd (28.5%) | 12 | ||||
| neuD | 13984–14610 | 209 | Acetyltransferaseb | Escherichia coliNeuDd (33.0%) | 1 |
| neuA | 14624–15862 | 413 | CMP-sialic acid synthetaseb | Escherichia coliNeuAc (33.0%) | 52 |
| Streptococcus agalactiae type III CpsFc (99.2%) | 17 | ||||
| orf1 | 15979–16461 | 161 | Unknown | Escherichia coliEvgSde (28.2%) | 43 |
| ung | 16563–17213 | 217 | Uracil DNA glycosylaseb | Streptococcus pneumoniaeUngd (75.8%) | 31 |
| Bacillus subtilisUngd (52.6%) | 13 |
a
Experimentally determined in this study.
b
Predicted by sequence similarity.
c
Enzymatic activities have been determined.
d
Enzymatic activity was proposed.
e
Homology refers to the N-terminal part of the gene product.
f
Homology refers to the C-terminal part of the gene product.