TABLE 2.
Strains, phage, and plasmids used in this study
| Strain, phage, or plasmid | Genotype | Reference or source |
|---|---|---|
| E. coli strains | ||
| ECL116 | F− ΔlacU169 endA hsdR thi | 1 |
| SME1082 | ECL116 ΔrhaSa | Laboratory collection |
| SME1212 | SME1082 λSME103 C(−46)Gb | This study |
| SME1213 | SME1082 λSME103 T(−78)Gb | This study |
| SME1214 | SME1082 λSME103 C(−65)Tb | This study |
| SME1221 | SME1082 λSME103 T(−77)Gb | This study |
| SME1222 | SME1082 λSME103 wtc | This study |
| SME1724 | SME1082 λSME103 G(−48)Tb | This study |
| SME1725 | SME1082 λSME103 A(−81)Cb | This study |
| SME1726 | SME1082 λSME103 G(−67)Ab | This study |
| SME1733 | SME1082 λSME103 C(−79)Ab | This study |
| Phage | ||
| λRS45 | bla′-lacZscatt+ int+ imm21 | 34 |
| λSME103 | λRS45 Φ(rhaB-lacZ)Δ110 | 10 |
| Plasmids | ||
| pRS414 | Apr, ′lacZ lacY+ lacA+ | 34 |
| pALTER-1 | Aps TetrlacZ f1 ori | Promega Corp. |
| pSE101 | AprrhaBAD+ rhaSR+ | 10 |
| pSE105 | pRS414 Φ(rhaB-lacZ) (wt) | 10 |
| pSE159 | Apr pALTER-1 rhaS (wt) | This study |
| pSE160 | Tetr pALTER-1 rhaS (wt) | This study |
| pSE165 | pSE160 (Leu201Ala) | This study |
| pSE166 | pSE159 (Arg202Ala) | This study |
| pSE167 | pSE159 (His205Ala) | This study |
| pSE168 | pSE160 (Arg206Ala) | This study |
| pSE169 | pSE159 (Cys246Ala) | This study |
| pSE170 | pSE160 (Gly247Ala) | This study |
| pSE171 | pSE159 (Phe248Ala) | This study |
| pSE172 | pSE160 (Asp250Ala) | This study |
| pSE173 | pSE159 (Asn252Ala) | This study |
| pSE174 | pSE159 (His253Ala) | This study |
| pSE175 | pSE159 (Phe254Ala) | This study |
| pSE176 | pSE159 (Ser255Ala) | This study |
| pSE177 | pSE159 (Thr256Ala) | This study |
| pSE178 | pSE159 (Gly247Arg) | This study |
| pSE179 | pSE160 (Phe248Val) | This study |
| pSE180 | pSE160 (Ser249Gly) | This study |
| pSE181 | pSE160 (Ser251Gly) | This study |
| pSE182 | pSE159 (Asn252His) | This study |
| pSE183 | pSE159 (Asn252Ser) | This study |
| pSE184 | pSE160 (Asn252Ile) | This study |
| pSE185 | pSE160 (His253Gln) | This study |