Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Recombinant DNA reagent | pGEX-TEV | Choi et al., 2012 | Ampicillin resistance; expression in bacterial cultures; pGEX-KG plasmid (ATCC) with a new TEV protease site Contact Weis lab for distribution |
|
| Strain, strain background (Escherichia coli) | BL21 (DE3) Codon-Plus RIL | Agilent | 230245 | Strain for expressing recombinant proteins |
| Chemical compound, drug | Halo-tag ligand Succinimidyl Ester O4 | P6741 | Used for labeling GFP to attach to halotag constructs | |
| Chemical compound, drug | Biotin-NHS | Millipore Sigma | P203118 | Labeling actin filaments |
| Chemical compound, drug | Rhodamine phallodin | Cytoskeleton | PHDR1 | Visualizing actin filaments |
| Chemical compound, drug | Trolox | Fischer Scientific | AC218940010 | |
| Chemical compound, drug | Bovine serum albumin, BSA | MCLAB | UBSA-100 | |
| Other | Streptavidin-coated polystyrene microspheres |
Bangs Laboratories, Inc | CP01004 | |
| Software, algorithm | UCSF Chimera 1.14 | Pettersen et al., 2004 | RRID:SCR_004097 | |
| Software algorithm | Python 3.9.1 | https://www.python.org/ | RRID:SCR_008394 | |
| Software algorithm | NumPy (v. 1.20.2) | https://numpy.org | RRID:SCR_008633 | Python library |
| Software algorithm | Pandas (v.1.3.1) |
https://pandas.pydata.org | RRID:SCR_018214 | Python library |
| Software algorithm | SciPy (v.1.6.2) |
https://scipy.org | RRID:SCR_008058 | Python library |
| Software algorithm | Matplotlib (v.3.5.1) |
http://matplotlib.sourceforge.net | RRID:SCR_008624 | Python library |