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. 2022 Aug 24;11:e78385. doi: 10.7554/eLife.78385

Table 2. Yeast strains used in this study.

ID Genotype Source
KWY165 haploid, MATa leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15 [phi+] W303 haploid
KWY166 diploid, leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15 [phi+] W303 diploid
KWY1602 MAT(alpha) his3-1 leu2-0 lys2-0 ura3-0 BY4742
KWY9200 KWY165, BRL1::BRL1-V5-IAA7-KanMX trp1::pADH1-dsRED-HDEL-TRP1 This study
KWY9204 KWY165, BRL1::BRL1-V5-IAA7-KanMX his3::pGPD1-OsTIR1 trp1::pADH1-dsRED-HDEL-TRP1 This study
KWY9268 KWY165, BRL1::BRL1-V5-IAA7-KanMX trp1::pADH1-dsRED-HDEL-TRP1 NUP82::NUP82-yEGFP-URA3 This study
KWY9269 BRL1::BRL1-V5-IAA7-KanMX his3::pGPD1-OsTIR1 trp1::pADH1-dsRED-HDEL-TRP1 NUP82::NUP82-yEGFP-URA3 This study
KWY9270 KWY165, BRL1::BRL1-V5-IAA7-KanMX trp1::pADH1-dsRED-HDEL-TRP1 NUP116::NUP116-yEGFP-URA3 This study
KWY9271 KWY165, BRL1::BRL1-V5-IAA7-KanMX his3::pGPD1-OsTIR1 trp1::pADH1-dsRED-HDEL-TRP1 NUP116::NUP116-yEGFP-URA3 This study
KWY9272 KWY165, BRL1::BRL1-V5-IAA7-KanMX trp1::pADH1-dsRED-HDEL-TRP1 NUP100::NUP100-yEGFP-URA3 This study
KWY9273 KWY165, BRL1::BRL1-V5-IAA7-KanMX his3::pGPD1-OsTIR1 trp1::pADH1-dsRED-HDEL-TRP1 NUP100::NUP100-yEGFP-URA3 This study
KWY9274 KWY165, BRL1::BRL1-V5-IAA7-KanMX trp1::pADH1-dsRED-HDEL-TRP1 NUP133::NUP133-yEGFP-URA3 This study
KWY9275 KWY165, BRL1::BRL1-V5-IAA7-KanMX his3::pGPD1-OsTIR1 trp1::pADH1-dsRED-HDEL-TRP1 NUP133::NUP133-yEGFP-URA3 This study
KWY9276 KWY165, BRL1::BRL1-V5-IAA7-KanMX trp1::pADH1-dsRED-HDEL-TRP1 NUP170::NUP170-yEGFP-URA3 This study
KWY9277 KWY165, BRL1::BRL1-V5-IAA7-KanMX his3::pGPD1-OsTIR1 trp1::pADH1-dsRED-HDEL-TRP1 NUP170::NUP170-yEGFP-URA3 This study
KWY9908 KWY165, BRL1::BRL1-V5-IAA7 KanMX trp1::ADH1-dsRED-HDEL-TRP1 NUP60::NUP60-yEGFP-URA3 This study
KWY10108 KWY165, BRL1::BRL1-V5-IAA7 KanMX his3::pGPD1-OsTIR1 trp1::pADH1-dsRED-HDEL-TRP1 NUP60::NUP60-yEGFP-URA3 This study
KWY9909 KWY165, BRL1::BRL1-V5-IAA7 KanMX trp1::pADH1-dsRED-HDEL-TRP1 NUP159::NUP159-yEGFP-URA3 This study
KWY10109 KWY165, BRL1::BRL1-V5-IAA7 KanMX his3::pGPD1-OsTIR1 trp1::pADH1-dsRED-HDEL-TRP1 NUP159::NUP159-yEGFP-URA3 This study
KWY9911 KWY165, BRL1::BRL1-V5-IAA7 KanMX trp1::pADH1-dsRED-HDEL-TRP1 MLP1::MLP1-yEGFP-URA3 This study
KWY10110 KWY165, BRL1::BRL1-V5-IAA7 KanMX his3::pGPD1-OsTIR1 trp1::pADH1-dsRED-HDEL-TRP1 MLP1::MLP1-yEGFP-URA3 This study
KWY9939 KWY165, BRL1::BRL1-V5-IAA7 KanMX trp1::pADH1-dsRED-HDEL-TRP1 NUP85::NUP85-yEGFP-URA3 This study
KWY10107 KWY165, BRL1::BRL1-V5-IAA7 KanMX his3::pGPD1-OsTIR1 trp1::pADH1-dsRED-HDEL-TRP1 NUP85::NUP85-yEGFP-URA3 This study
KWY10029 KWY165, BRL1::BRL1-V5-IAA7 KanMX trp1::pADH1-dsRED-HDEL-TRP1 NUP192::NUP192-yEGFP-URA3 This study
KWY10111 KWY165, BRL1::BRL1-V5-IAA7 KanMX his3::pGPD1-OsTIR1 trp1::pADH1-dsRED-HDEL-TRP1 NUP192::NUP192-yEGFP-URA3 This study
KWY5540 KWY165, nup116ΔGLFG NUP188::HIS3MX-pMet3-3xHA-Nup188 Onischenko et al., 2017
KWY8891 KWY165, ura3::pGAL1-BRL1-yeGFP-CaURA3 This study
KWY8893 KWY165, ura3::pGAL1-BRL1Δah-yeGFP-CaURA3 This study
KWY9070 KWY165, ura3::pGAL1-BRL1(I395D)-yeGFP-CaURA3 This study
KWY8894 KWY165, ura3::pGAL1-MRATSK-CaURA3 This study
KWY8895 KWY165, ura3::pGAL1-BRL1-CaURA3 This study
KWY8896 KWY165, ura3::pGAL1-BRL1Δah-CaURA3 This study
KWY8898 KWY165, ura3::pGAL1-BRL1(I395D)-CaURA3 This study
KWY10154 KWY165, NUP82::NUP82-yeGFP-CaURA3 leu2::pRS305-LEU2 This study
KWY10155 KWY165, NUP82::NUP82-yeGFP-CaURA3 leu2::pGAL1-BRL1-mCherry-LEU2 This study
KWY10159 KWY165, NUP82::NUP82-yeGFP-CaURA3 leu2::pGAL1-BRL1(I395D)-mCherry-LEU2 This study
KWY10161 KWY165, NUP116::NUP116-yeGFP-CaURA3 leu2::pRS305-LEU2 This study
KWY10162 KWY165, NUP116::NUP116-yeGFP-CaURA3 leu2::pGAL1-BRL1-mCherry-LEU2 This study
KWY10166 KWY165, NUP116::NUP116-yeGFP-CaURA3 leu2::pGAL1-BRL1(I395D)-mCherry-LEU2 This study
KWY10168 KWY165, NUP133::NUP133-yeGFP-CaURA3 leu2::pRS305-LEU2 This study
KWY10169 KWY165, NUP133::NUP133-yeGFP-CaURA3 leu2::pGAL1-BRL1-mCherry-LEU2 This study
KWY10173 KWY165, NUP133::NUP133-yeGFP-CaURA3 leu2::pGAL1-BRL1(I395D)-mCherry-LEU2 This study
KWY10175 KWY165, NUP170::NUP170-yeGFP-CaURA3 leu2::pRS305-LEU2 This study
KWY10176 KWY165, NUP170::NUP170-yeGFP-CaURA3 leu2::pGAL1-BRL1-mCherry-LEU2 This study
KWY10180 KWY165, NUP170::NUP170-yeGFP-CaURA3 leu2::pGAL1-BRL1(I395D)-mCherry-LEU2 This study
KWY10260 KWY165, SEC61::SEC61-yEGFP-CaURA3 leu2-3::pRS305-LEU2 This study
KWY10261 KWY165, SEC61::SEC61-yEGFP-CaURA3 leu2-3::BRL1-mCherry-LEU2 This study
KWY10262 KWY165, SEC61::SEC61-yEGFP-CaURA3 leu2-3::BRL1(I395D)-mCherry-LEU2 This study
KWY8876 KWY165, nup116ΔGLFG NUP188::HIS3MX-pMet3-3xHA-Nup188 ura3::pGAL1-BRL1Δah-URA3 This study
KWY8874 KWY165, nup116ΔGLFG NUP188::HIS3MX-pMet3-3xHA-Nup188 ura3::pGAL1-BRL1-URA3 This study
KWY8877 KWY165, nup116ΔGLFG NUP188::HIS3MX-pMet3-3xHA-Nup188 ura3::pGAL1-MRATSK-URA3 This study
KWY8882 KWY165, nup116ΔGLFG NUP188::HIS3MX-pMet3-3xHA-Nup188 ura3::pGAL1-BRL1(F391D)-URA3 This study
KWY8883 KWY165, nup116ΔGLFG NUP188::HIS3MX-pMet3-3xHA-Nup188 ura3::pGAL1-BRL1(I395D)-URA3 This study
KWY8884 KWY165, nup116ΔGLFG NUP188::HIS3MX-pMet3-3xHA-Nup188 ura3::pGAL1-BRL1(F398D)-URA3 This study
KWY8885 KWY165, nup116ΔGLFG NUP188::HIS3MX-pMet3-3xHA-Nup188 ura3::pGAL1-BRL1(L402D)-URA3 This study
KWY8886 KWY165, nup116ΔGLFG NUP188::HIS3MX-pMet3-3xHA-Nup188 ura3::pGAL1-BRL1(D393A)-URA3 This study
KWY8887 KWY165, nup116ΔGLFG NUP188::HIS3MX-pMet3-3xHA-Nup188 ura3::pGAL1-BRL1(D400A)-URA3 This study
KWY9079 KWY165, trp1::dsRed-HDEL-TRP1 ura3::pGAL1-ahBRL1-yEGFP-CaURA3 This study
KWY9075 KWY165, trp1::dsRed-HDEL-TRP1 ura3::pGAL1-yEGFP-CaURA3 This study
KWY10418 KWY165, Brl1-mCherry::Nat his3::CRE-EBD78 NUP170::NUP170-loxP-GFP_URA3-loxP_STOP This study
KWY10419 KWY165, Brl1-mCherry::Nat his3::CRE-EBD78 NUP170::NUP170-loxP-STOP_URA3-loxP_GFP This study
KWY9964 KWY1602, BRL1::BRL1-S-TEV-ZZ-KanMX This study
KWY10453 KWY1602, BRL1::BRL1-V5-IAA7-KanMX his3::pGPD1-OsTIR1 NUP170::NUP170-S-TEV-ZZ-URA3 This study
KWY10241 KWY1602, BRL1::BRL1-V5-IAA7-KanMX his3::pGPD1-OsTIR1 This study
KWY10697 KWY1602, BRL1::BRL1-V5-IAA7-KanMX his3::pGPD1-OsTIR1 ura3::Nup82-yEGFP leu2-3::BRL1(I395D)-mCherry-LEU2 This study
KWY10445 KWY1602, BRL1::BRL1-V5-IAA7-KanMX leu2::pGPD1-OsTIR1 MLP1::MLP1-V5-loxP-mCherry_HygMX-loxP_GFP his3::CRE-EBD78 This study
KWY10450 KWY1602, BRL1::BRL1-V5-IAA7-KanMX leu2::pGPD1-OsTIR1 NUP82::NUP82-V5-loxP-mCherry_HygMX-loxP_GFP his3::CRE-EBD78 This study
KWY10451 KWY1602, BRL1::BRL1-V5-IAA7-KanMX leu2::pGPD1-OsTIR1 NUP188::NUP188-V5-loxP-mCherry_HygMX-loxP_GFP his3::CRE-EBD78 This study
KWY10452 KWY1602, BRL1::BRL1-V5-IAA7-KanMX leu2::pGPD1-OsTIR1 NUP133::NUP133-V5-loxP-mCherry_HygMX-loxP_GFP his3::CRE-EBD78 This study
KWY9485 KWY166, BRL1/brl1Δah This study
KWY9489 KWY166, BRL1/brl1(I395D) This study