Figure 8.

Age-dependent increases in hypophosphorylated neurofilaments in Atl1^KI/KI/Reep1^−/− mice. (A) Lysates of spinal cord from WT and Atl1^KI/KI/Reep1^−/− mice of the indicated ages were immunoblotted for total and hypophosphorylated NFH. GAPDH levels were monitored as a control for protein loading. (B-D) Quantifications of immunoblots as in A for NFH/GAPDH (B), hypophosphorylated-NFH/GAPDH (C), and hypophosphorylated NFH/NFH (D). Two-way ANOVA (Tukey’s test) was applied. n = 4. ^*P < 0.05, ^**P < 0.01, ^***P < 0.005, ^****P < 0.001.