Figure 2.

Behavioral changes in Atl1^KI/KI/Reep1^−/− mice. (A) Representative images of treadmill tests. An 8-month old female Atl1^KI/KI/Reep1^−/− mouse is unable to use its hind paws while ambulating, in contrast to the WT mouse. (B) Atl1^KI/KI/Reep1^−/− mice progressively lose the ability to run on the treadmill. (C) Atl1^KI/KI/Reep1^−/− mice fall from the rotarod much earlier than WT mice. (D) Ledge tests show that Atl1^KI/KI/Reep1^−/− mice have poor ability to walk on cage ledge. (E) Hindlimb clasping is observed in Atl1^KI/KI/Reep1^−/− mice starting from 8 weeks. n = 8 for WT male, Atl1^KI/KI/Reep1^−/− male, and Atl1^KI/KI/Reep1^−/− female; n = 9 for WT female. All data were analyzed by two-way ANOVA (Tukey’s test). ^***P < 0.005, ^****P < 0.001.