Table 1.
Details of antibodies used.
| Antibody | Source | Dilution |
|---|---|---|
| NeuN (rabbit) | Abcam (ab177487) | 1 : 2000 (WB), 1 : 200 (IF) |
| NeuN (mouse) | Abcam (ab104224) | 1 : 2000 (WB), 1 : 200 (IF) |
| Synapsin (rabbit) | Abcam (ab64581) | 1 : 1000 (WB) |
| Neurofilament (rabbit) | Abcam (ab207176) | 1 : 1000 (WB), 1 : 200 (IF, IHC) |
| β-Actin (mouse) | Santa Cruz (sc-47778) | 1 : 3000 (WB) |
| Lectin | VectorLab (B-1175-1) | 1 : 500 (IHC), 1 : 200 (IF) |
| PDGFRβ (mouse) | Abcam (ab69506) | 1 : 2000 (WB), 1 : 200 (IF) |
| CD34 (rabbit) | Abcam (ab81289) | 1 : 200 (IF) |
| CD45 (rabbit) | Abcam (ab40763) | 1 : 1000 (WB), 1 : 200 (IF) |
| Fibronectin (rabbit) | Abcam (ab268020) | 1 : 100 (IF) |
| F4/80 (rat) | Abcam (ab6640) | 1 : 1000 (WB), 1 : 200 (IF, IHC) |
| PDGFB (rabbit) | Abcam (ab23914) | 1 : 1000 (WB), 1 : 200 (IF) |
| VEGFA (rabbit) | Abcam (ab39250) | 1 : 1000 (WB), 1 : 200 (IF) |
| HRP-goat anti-mouse | Santa Cruz (sc-2973) | 1 : 4000 (WB) |
| HRP-goat anti-rabbit | Santa Cruz (sc-2357) | 1 : 4000 (WB) |
| HRP-goat anti-rat | Santa Cruz (sc-362293) | 1 : 4000 (WB) |
| AMCA-streptavidin | VectorLab (SA-5008-1) | 1 : 200 (IF) |
| Alexa Fluor 488 goat anti-rabbit | Jackson (111-545-144) | 1 : 200 (IF) |
| Alexa Fluor 568 goat anti-mouse | Jackson (115-545-166) | 1 : 200 (IF) |
| Alexa Fluor 488 donkey anti-mouse | Jackson (715-545-020) | 1 : 200 (IF) |
| Alexa Fluor 594 goat anti-rabbit | Jackson (111-545-003) | 1 : 200 (IF) |
| Alexa Fluor 594 goat anti-rat | Jackson (112-585-003) | 1 : 200 (IF) |
WB: Western blot; IHC: immunocytochemistry; IF: immunofluorescence.