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. 2022 Jul 13;608(7924):819–825. doi: 10.1038/s41586-022-04930-8

Fig. 3. C-strand synthesis overcomes GQ structures in the template DNA.

Fig. 3

a, Mutating the telomeric repeat sequence (noGQ) greatly increases C-strand synthesis (compare lane 4 with lane 5 and lane 7 with lane 8). The GC tail templates have 10-nt GC sequences added on their 5′ ends to test whether the C-strand synthesis proceeds to the end of the template (compare lanes 4 and 6). Although 3×TEL is inactive, adding the 10-nt tail to 3×TEL provides a template for C-strand synthesis (lane 2). b, C-strand synthesis when GQ structures are destabilized (LiCl) or stabilized (KCl). NaCl is an intermediate condition. CST–Polα–primase at 25 nM; DNA templates at 100 nM. c, Activity with 100 mM LiCl relative to that in 100 mM KCl or activity with 100 mM NaCl relative to that with 100 mM KCl; quantification of two experiments including the one in b. *P < 0.0001, two-tailed t-test; n = 6 independent experiments for the noGQ templates and n = 8 independent experiments for the GQ templates. For gel source data, see Supplementary Fig. 1.