Extended Data Fig. 3. Processivity of CST–Polα-primase on poly(dT) templates.
a, Reactions as in Fig. 2b but with increasing concentrations of the indicated templates. Products labelled with α-32P-dATP. LC, loading control. Arrows, rare extension to the ends of these templates. Note that the templates containing 3xTEL give much more product than poly(dT), but the length of the products (which at high [template] is limited by processivity) is similar for all templates. b, The intensity of each extension product (that is, the counts in each band of a lane in a) was divided by the number of As in that product and the median length was calculated. Diamonds, 3xTELdT72. Squares, 3xTELdT54. Circles, dT72. The observation that the median extension is largely independent of the template DNA concentration means that extension is limited by the processivity of the enzyme on that template. For gel source data, see Supplementary Fig. 1