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. 2021 Sep 19;75(1):e792–e804. doi: 10.1093/cid/ciab823

Figure 4.

Figure 4.

Evaluation of cellular immune response through ELISPOT upon stimulation with Mega Pools of peptides derived from SARS-CoV-2 proteins in volunteers immunized with CoronaVac. Numbers of IFN-γ-secreting cells, determined through ELISPOT as spot forming cells (SFCs) were determined. (A) Representative pictures for each stimulus are shown. PBMCs were stimulated with (B) MP-S, (C) MP-R, (D) MP-S + R, (E) MP-CD8A, (F) MP-CD8B, and (G) MP-CD8A + B for 48 h for samples obtained before the first dose, and 2 (second dose + 2 weeks) and 4 weeks (second dose + 4 weeks) after the second dose. A total of 47 volunteers were evaluated for MP-S and MP-R and 27 volunteers for MP-CD8A and MP-CD8B. Data shown represent median ± 95% CI. Statistical differences were evaluated by a Friedman test for repeated measures, followed by a post hoc Dunn test corrected for multiple comparisons against day preimmune samples; n.s. = no statistical differences, *P < .05, **P < .005. Abbreviations: CI, confidence interval; ELISPOT, enzyme-linked immunospot; IFN, interferon; MP, Mega Pools; PBMC, peripheral blood mononuclear cell; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2.