FIG. 2.

Analysis of RNA association with the Era protein of S. pneumoniae. E. coli rRNAs were isolated as described in Materials and Methods. All samples were electrophoresed on a 1.5% agarose gel containing ethidium bromide. Lane 1, DNA standards (100-bp increments from bottom to top; Gibco BRL); lanes 2 and 3, E. coli rRNA untreated and treated with RNase A, respectively; lanes 4 and 5, phenol-chloroform-extracted material from a purified GST-Era protein preparation untreated and treated with RNase A, respectively; lanes 6 and 7, a purified GST-Era protein preparation untreated and treated with RNase A, respectively; lane 8, phenol-chloroform-extracted material from a purified GST-Era protein preparation treated with DNase.