. 2022 Aug 11;13:928683. doi: 10.3389/fgene.2022.928683

TABLE 2.

Summary of product sequences obtained after protease digestion of the FBN1^E2cbEGF1 peptides, FBN1^{E2cbEGF1+C160Y} peptides, FBN1^cbEGF20-24 peptides, and FBN1 ^{cbEGF20-24+C1350Y} peptides.

Peptide	Protease	Peptide	Fragment
FBN1^E2cbEGF1/ FBN1^{E2cbEGF1+C160Y}	Trypsin	S¹¹⁵IQHCNIR¹²²	Both
		G¹³⁹YIGTHCGQPVCESGCLNGGR¹⁵⁹	Both
		C¹⁶⁰VAPNR¹⁶⁵	FBN1^E2cbEGF1
		C¹⁶⁰VAPNRCACTYGFTGPQCER¹⁷⁹	FBN1^E2cbEGF1
		Y¹⁶⁰VAPNR¹⁶⁵	FBN1^{E2cbEGF1+C160Y}
		C¹⁶⁶ACTYGFTGPQCER¹⁷⁹	Both
		T¹⁸³GPCFTVISNQMCQGQLSGIVCTK²⁰⁶	Both
		T²⁰⁷LCCATVGR²¹⁵	Both
		R²³³GFIPNIR²⁴⁰	Both
		G²³⁴FIPNIR²⁴⁰	Both
		L²⁷⁹NEVSQK²⁸⁵	Both
	Chymotrypsin	C¹³⁶QKGY¹⁴⁰	FBN1^E2cbEGF1
		C¹³⁶QKGYIGTHCGQPVCESGCLNGGRY¹⁶⁰	FBN1^{E2cbEGF1+C160Y}
		I¹⁴¹GTHCGQPVCESGCLNGGRCVAPNRCACTY¹⁷⁰	FBN1^E2cbEGF1
		V¹⁶¹APNRCACTY¹⁷⁰	FBN1^{E2cbEGF1+C160Y}
		G¹⁷¹FTGPQCERDY¹⁸¹	Both
		G¹⁷¹FTGPQCERDYRTGPCF¹⁸⁷	Both
		R¹⁸²TGPCF¹⁸⁷	Both
		S²⁰⁰GIVCTKTL²⁰⁸	Both
		C²⁰⁹CATVGRAW²¹⁷	Both
		A²¹¹TVGRAW²¹⁷	Both
		I²³⁶PNIRTGACQDVDECQAIPGL²⁵⁶	Both
		C²⁵⁷QGGNCINTVGSF²⁶⁹	Both
		C²⁵⁷QGGNCINTVGSFECKCPAGHKLNEVSQKCEL²⁸⁸	FBN1^E2cbEGF1
		E²⁷⁰CKCPAGHKL²⁷⁹	Both
FBN1^cbEGF20-24/ FBN1 ^{cbEGF20-24+C1350Y}	Trypsin	T¹²⁵⁵NIPGEYR¹²⁶²	Both
		C¹²⁶³LCYDGFMASEDMK¹²⁷⁶	Both
		T¹²⁷⁷CVDVNECDLNPNICLSGTCENTK¹³⁰⁰	Both
		G¹³⁰¹SFICHCDMGYSGK¹³¹⁴	Both
		T¹³¹⁸GCTDINECEIGAHNCGK¹³³⁵	Both
		H¹³³⁶AVCTNTAGSFK¹³⁴⁷	Both
		C¹³⁴⁸SCSPGWIGDGIK¹³⁶⁰	FBN1^cbEGF20-24
		C¹³⁴⁸SYSPGWIGDGIK¹³⁶⁰	FBN1^{cbEGF20-24+C1350Y}
		N¹³⁸²TMGSYR¹³⁸⁸	Both
		N¹⁴¹⁷GQCLNAPGGYR¹⁴²⁸	Both
		C¹⁴²⁹ECDMGFVPSADGK¹⁴⁴²	Both
	Chymotrypsin	M¹²⁷⁰ASEDMKTCVDVNECDL¹²⁸⁶	Both
		S¹²⁹³GTCENTKGSF¹³⁰³	Both
		I¹³⁰⁴CHCDMGY¹³¹¹	Both
		H¹³³⁶AVCTNTAGSF¹³⁴⁶	Both
		K¹³⁴⁷CSCSPGW¹³⁵⁴	FBN1^cbEGF20-24
		S¹³⁵¹PGW¹³⁵⁴	FBN1^{cbEGF20-24+C1350Y}
		I¹³⁵⁵GDGIKCTDL¹³⁶⁴	Both
		A¹³⁷⁸DCKNTMGSY¹³⁸⁷	Both
		R¹³⁸⁸CLCKEGY¹³⁹⁵	Both
		T¹³⁹⁶GDGF¹⁴⁰⁰	Both
		N¹⁴¹³LCGNGQCLNAPGGY¹⁴²⁷	Both
		R¹⁴²⁸CECDMGF¹⁴³⁵	Both

FBN1, fibrillin-1; cbEGF, calcium-binding epidermal growth factor.