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. 2022 Aug 11;9:889667. doi: 10.3389/fmolb.2022.889667

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Copyright © 2022 Paliwal, Thom, Hussein, Ravishankar, Wilkes, Charleston and Jones.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rescue of soluble purified recombinant bTB proteins that were originally poorly expressed by alternate positioning of the His tag. Panel (A). Examples of N-terminal tagged proteins purified from E.coli. 1- Mb3338 (31 kDa), 2-Mb2656 (31 kDa), 3-Mb2898 (22 kDa), 4-Mb2900 (19 kDa), 5-Mb0130 (34 kDa), 6-Mb0854 (31 kDa), 7- Mb1656 (53 kDa), 8-Mb3652 (18 kDa), 9-Mb3871 (20 kDa). Panel (B). Examples of N-terminal tagged proteins purified from insect cells. 1- Mb0977_Mb3046c (43 kDa), 2-Mb0959 (39 kDa), 3-Mb1319 (44 kDa), 4-Mb3070 (37 kDa), 5-Mb2244 (53 kDa), 6-Mb1228 (39 kDa). Molecular weight markers (M) are labelled to the left of each panel and are in kilodaltons.