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. 2022 Aug 11;9:889667. doi: 10.3389/fmolb.2022.889667

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Copyright © 2022 Paliwal, Thom, Hussein, Ravishankar, Wilkes, Charleston and Jones.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rescued expression of problematic bTB proteins in E.coli by non-His fusion tags. Panel (A). The secretion accessory proteins EspC and EspA. 1- C-terminally His tagged EspC, 2- N-terminally GFP tagged EspA. Panel (B). 1-GB_Mb1762 (29 kDa), 2-GB_Mb1916 (25 kDa), 3-GB_Mb2410 (22 kDa) all expressed and purified as N-terminal fusions with the B1 domain of streptococcal protein G. Molecular weight markers (M) are labelled to left of each panel and are in kilodaltons.