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. 1999 Sep;181(17):5257–5262. doi: 10.1128/jb.181.17.5257-5262.1999

TABLE 1.

Plasmids used in this study

Plasmid Description Source or reference
pPOT1 pGEX-derived plasmid used to clone the library of rRNA gene fragments 44; transcription of the RNA fragment (from Ptac) is IPTG inducible
pA750 pPOT1, from the library, containing the 32-nucleotide rDNA fragment whose transcript is complementary to nucleotides 735 to 766 of 23S rRNA This work
pS750 pPOT1 with the A750 insert in the opposite transcriptional orientation, yielding a transcript corresponding to the direct sequence of nucleotides 735 to 766 of 23S rRNA This work
pACYC184 Contains the wild-type cat gene 14
pCAT101 pACYC184 derivative containing the cat gene with a UGA nonsense mutation at codon 74 This work
pCAT102 pACYC184 derivative containing the cat gene with a UAA nonsense mutation at codon 74 This work
pCAT103 pACYC184 derivative containing the cat gene with a UAG nonsense mutation at codon 74 This work
pNC160 Randomly chosen plasmid from the rRNA fragment library which contains a 160-nucleotide rRNA gene fragment whose transcript is complementary to nucleotides 377 to 537 of 23S rRNA This work