pPOT1 |
pGEX-derived plasmid used to clone the library of rRNA gene fragments |
44; transcription of the RNA fragment (from Ptac) is IPTG inducible |
pA750 |
pPOT1, from the library, containing the 32-nucleotide rDNA fragment whose transcript is complementary to nucleotides 735 to 766 of 23S rRNA |
This work |
pS750 |
pPOT1 with the A750 insert in the opposite transcriptional orientation, yielding a transcript corresponding to the direct sequence of nucleotides 735 to 766 of 23S rRNA |
This work |
pACYC184 |
Contains the wild-type cat gene |
14 |
pCAT101 |
pACYC184 derivative containing the cat gene with a UGA nonsense mutation at codon 74 |
This work |
pCAT102 |
pACYC184 derivative containing the cat gene with a UAA nonsense mutation at codon 74 |
This work |
pCAT103 |
pACYC184 derivative containing the cat gene with a UAG nonsense mutation at codon 74 |
This work |
pNC160 |
Randomly chosen plasmid from the rRNA fragment library which contains a 160-nucleotide rRNA gene fragment whose transcript is complementary to nucleotides 377 to 537 of 23S rRNA |
This work |