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Levels of CpxR and CpxA are elevated in a constitutively activated cpx* background. Western blots were performed on whole-cell lysates (A and C) or membrane fractions (B) of MC4100 (lane 1), TR51 (A and C, lane 2), TR8 (B, lane 2), or TR10 (lane 3) bacteria by using polyclonal antisera directed against MBP-CpxR (A and C) or MBP-CpxA (B) fusion proteins.
