TABLE 2.

Retention time, linearity, and sensitivity for each steroid.

Steroid	Retention time (min)	Linearity			Sensitivity (ng/ml)
		Range (ng/ml)	Equation	r 2	LoD (S/N ≥ 3)	LLoQ (S/N ≥ 10)
Aldosterone	1.69	0.1–20	y = 0.9352x − 0.0133	0.9989	0.01	0.05
18OH coryicosterone	1.83	0.1–20	y = 0.0719x + 0.0031	0.9982	0.05	0.1
Cortisone	2.06	1.5–300	y = 0.0479x + 0.0082	0.9996	0.1	1.0
Cortisol	2.21	1.5–300	y = 0.0448x − 0.0078	0.9991	0.2	1.0
21-Deoxycortisol	2.79	0.1–20	y = 0.8178x + 0.0322	0.9967	0.05	0.1
Corticosterone	3.04	0.1–20	y = 0.6865x + 0.0066	0.9997	0.01	0.05
11-Deoxycortisol	3.19	0.1–20	y = 0.8070x + 0.0156	0.9996	0.01	0.05
Androstenedione	3.68	0.1–20	y = 0.2282x + 0.0027	0.9988	0.01	0.05
Deoxcorticosterone	3.98	0.1–20	y = 1.0350x + 0.0001	0.9996	0.01	0.05
Testosterone	4.05	0.1–20	y = 0.2941x − 0.0018	0.9998	0.01	0.05
Androstenediol	4.06	1.5–300	y = 0.0045x + 0.0087	0.9952	1.0	1.5
DHEA	4.38	15–3,000	y = 0.0004x + 0.0014	0.9995	4.0	15
17-OHP	4.40	0.1–20	y = 0.4351x + 0.0037	0.9995	0.01	0.05
17-hydroxy pregnenolone	4.45	1.5–300	y = 0.0068x + 0. 0034	0.9985	0.5	1.0
Dihydrotestosterone	5.36	0.1–20	y = 0.6425x − 0.0044	0.9995	0.01	0.05
Progesterone	5.51	0.25–50	y = 0.0307x + 0.0012	0.9995	0.02	0.1
Pregnenolone	5.57	0.25–50	y = 0.0374x + 0.0125	0.9991	0.2	1.0