Table 4.

Detection of an artificially created mixed clone infection of two previously genotyped samples from the same period and area in Vietnam.

Ratio of mixed isolates	Mean depth	% of heterozygotes detected
		n detected of total variant loci	% variant loci detected
50:50	212.0	56/58	96.6%
80:20	192.5	56/58	96.6%
90:10	182.2	54/58	93.1%
95:5	197.3	44/58	75.9%
98:2	174.4	46/58	79.3%

The minority clone could be detected down to 2%, although at ≥5% some genotypes of the minority clone could no longer be detected.