FIG. 2.
Transcription start site of cysHRm. Primer extension experiments reveal two transcription start sites, SS1 and SS2. (a) Sequencing ladder (lanes 1 to 4), with primer extension reactions using Rm1021/pMW205 RNA grown in M9 minimal medium plus cysteine and methionine (lane 5) or M9 minimal medium alone (lane 6). (b) Region upstream of the ATG for cysHRm. The solid arrows represent SS1 and SS2, while the putative CysB binding site, the putative ribosome binding site (RBS), and the −10 and −35 regions are boxed and labeled. SS2 is upstream of the putative CysB binding site. (c) Structure of cysHDNRm. The small solid rectangle represents the putative CysB binding site.