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Inhibition of His6-CysHRm (0.312 mg/ml)-mediated reduction of 83 nM [35S]APS by 5′-AMP (○) but not by 3′-AMP (✻). The standard reaction mixture was incubated for 1 h at 30°C, heat killed at 95°C for 3 min, and analyzed on a polyethyleneimine-cellulose TLC plate developed in 1 M LiCl. The intensity of each spot was quantitated with a phosphoimager. The percent sulfite was calculated as [(amount of sulfite signal)/(amount of sulfite signal + APS signal)] × 100.
