Table 3.
Differences in lymph node processing methods and upstage rates of previous reports
|
Ref.
|
Subject (patients)
|
OSNA method
|
Harvested LN, n
|
Harvested LN, median
|
Dividing method of LN
|
Pathological staining
|
Measured LN by OSNA, n
|
Measured LN by OSNA, median
|
Upstage rate (pStage I and II)
|
| Yamamoto et al[16] | Stage 0, I (85) | cOSNA | 434 | N/Aa | Fourb; 4 mm over diameter of LN | HE and IHC | 385 | 4.5 | 16.5% (2/16) |
| Güller et al[12] | Stage I, II, III (22) | cOSNA | 313 | 30 (16–60) | Fourb; 3 mm over diameter of LN | HE and IHC | 56 | 13 (6–24) | 15.3% (2/13) |
| Croner et al[14] | Stage I, II (103) | cOSNA | N/Aa | N/Aa | Centerc; 6 mm over halfd; 4 mm to 6 mm diameter of LN | HE | 1594 | 14 (1–46) | 25.2% (26/103) |
| Vogelaar et al[15] | Stage I, II (128) | cOSNA | N/Aa | N/Aa | Fourb; 10 mm over halfd; 10 or less than 10 mm diameter of LN | HE and IHC | 317 | Mean 15.3 (4–40) | 20.2% (20/90) |
| Yamamoto et al[17] | Stage I, II, III (204) | cOSNA | 4324 | 19 (3–25) | Halfd | HE | 1925 | 8 (2–25) | 17.6% (13/74) |
| Aldecoa et al[133] | Stage I, II (149) | cOSNA | 2483 | 15 | Centerc | HE | 1940 | 12 | 51% (76/149) |
| Rakislova et al[125] | Stage I, II, III (188) | cOSNA, pOSNA | cOSNA 1828, pOSNA 1992 | cOSNA 17 (13-22), pOSNA 20.5 (17-27) | Centerc | HE | cOSNA 1757, pOSNA 1449 | cOSNA 13 (10-18), pOSNA 18 (13-25) | cOSNA 55.4% (51/92), pOSNA 20.7% (16/77) |
| Brito et al[126] | Stage I, II (59) | cOSNA | 1046 | 13 (9-19) | Centerc; 5 mm over halfd; 4 or less diameter of LN | HE | 753 | 12 (7-16) | 28.8% (17/59) |
| Itabashi et al[18] | Stage I, II, III (195) | cOSNA | Not shown | 19 (1-75) | Halfd; 4 mm over diameter of LN | HE | Not shown | 8 (2-25) | 15.7% (11/70) in stage II patients |
| Tani et al[33] | Stage II, IIIA (92) | pOSNA | 2236 | 24.3 (5–66) | Halfd; 4 mm over diameter of LN | HE | 636 | 6.9 (1–35) | 9.1% (6/66) |
a
Not applicable.
b
Four section method: dividing lymph nodes into four equal sections and sending two of these sections (50%) for pathology and one-step nucleic acid amplification (OSNA) measurement.
c
Center-cut method: 1 mm from the center of lymph nodes are sent for pathological examination and the rest are used for OSNA.
d
Half-division method divides lymph nodes in half and sends each 50% portion for pathology and OSNA.
HE: Hematoxylin and eosin; IHC: Immunohistochemistry; LN: Lymph node; OSNA: One-step nucleic acid amplification; pOSNA: Pooled one-step nucleic acid amplification.