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. 2022 Aug 21;13(3):19476035221118165. doi: 10.1177/19476035221118165

Figure 2.

Figure 2.

Identification of differentially expressed miRNAs and tissue-specific analysis. (A) Heatmap showing the differentially expressed miRNAs between chondrocytes at passages 1 and 5. (B) MA showing the differentially expressed miRNAs between chondrocytes at passages 1 and 5. (C) Volcano plot showing the differentially expressed miRNAs between chondrocytes at passages 1 and 5. (D) Principal component analysis showing the differentially expressed miRNAs between chondrocytes at passage 1 and passage 5. (E) qRT-PCR analysis of miRNA expression in chondrocytes at passage 1 compared to that at passage 5. (F) qRT-PCR analysis of miRNA expression in chondrocytes within passage 1 compared to that at passage 5. qRT-PCR analysis of (G) mmu-miR-145a-3p, (H) mmu-miR-132-5p, (I) mmu-miR-503-5p, and (J) mmu-miR-675-3p in different tissues. (K) β-Gal staining of chondrocytes at passage 5 with miRNA inhibitor NC or miR-132-5p inhibitor. qRT-PCR = real-time quantitative polymerase chain reaction; MA = minus-average; NC = negative control; CPM = count per million; PCA = principle component Analysis. **indicates P < 0.01 compared to passage 1.