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. 2022 Aug 21;13(3):19476035221118165. doi: 10.1177/19476035221118165

Figure 3.

Figure 3.

Identification of differentially expressed piRNAs and tissue-specific analysis. (A) Heatmap showing the differentially expressed piRNAs between chondrocytes at passages 1 and 5. (B) MA showing the differentially expressed piRNAs between chondrocytes at passages 1 and passage 5. (C) Scatter plot showing the differentially expressed piRNAs between chondrocytes at passages 1 and 5. (D) Volcano plot showing the differentially expressed piRNAs between chondrocytes at passages 1 and 5. (E) Principal components analysis showing the differentially expressed piRNAs between chondrocytes at passages 1 and 5. (F) qRT-PCR analysis of piRNA expression in chondrocytes within passage 1 compared to that at passage 5. qRT-PCR analysis of (G) mmu_piR_022877, (H) mmu_piR_000580, (I) mmu_piR_025007, and (J) mmu_piR_025576 in different tissues. (K) β-Gal staining of chondrocytes at passage 5 with piRNA mimics NC or piR_025576 mimics. qRT-PCR = real-time quantitative polymerase chain reaction; MA = minus-average; pLVX = plasmid lentiviral vector X; CPM = count per million; PCA = principle component analysis. * indicates P < 0.05 and ** indicates P < 0.01 compared to passage 1.