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. 2022 Aug 21;13(3):19476035221118165. doi: 10.1177/19476035221118165

Figure 4.

Figure 4.

Identification of differentially expressed snoRNAs and tissue-specific analysis. (A) Heatmap showing the differentially expressed snoRNAs between chondrocytes at passages 1 and 5. (B) MA showing the differentially expressed snoRNAs between chondrocytes at passages 1 and 5. (C) Volcano plot showing the differentially expressed snoRNAs between chondrocytes at passages 1 and 5. (D) Principal component analysis showing the differentially expressed snoRNAs between chondrocytes at passages 1 and 5. (E) qRT-PCR analysis of snoRNA expression in chondrocytes at passage 1 compared with that at passage 5. (F) qRT-PCR analysis of snoRNA expression in chondrocytes at passage 1 compared with that at passage 5. qRT-PCR analysis of (G) ENSMUSG00000087935, (H) ENSMUSG00000077604, (I) ENSMUSG00000095205, and (J) ENSMUSG00000065272 in different tissues. (K) β-Gal staining of chondrocytes at passage 5 with pLVX-Vecotor or pLVX-ENSMUSG00000087935. qRT-PCR = real-time quantitative polymerase chain reaction; MA = minus-average; pLVX = plasmid lentiviral vector X; CPM = count per million; PCA = principle component analysis. * indicates P < 0.05 and ** indicates P < 0.01 compared with passage 1.