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. 2022 Aug 21;13(3):19476035221118165. doi: 10.1177/19476035221118165

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© The Author(s) 2022

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — Identification of differentially expressed snRNAs and tissue-specific analysis. (A) Heatmap showing the differentially expressed snRNAs between chondrocytes at passages 1 and 5. (B) MA showing the differentially expressed snRNAs between chondrocytes at passages 1 and 5. (C) Scatter plot showing the differentially expressed snRNAs between chondrocytes at passages 1 and 5. (D) Volcano plot showing the differentially expressed snRNAs between chondrocytes at passages 1 and 5. (E) Principal components analysis showing the differentially expressed snRNAs between chondrocytes at passages 1 and 5. (F) qRT-PCR analysis of snRNA expression in chondrocytes at passage 1 compared with that at passage 5. qRT-PCR analysis of (G) ENSMUSG00000094655, (H) ENSMUSG00000064682, (I) ENSMUSG00000087754, and (J) ENSMUSG00000064923 in different tissues. (K) β-Gal staining of chondrocytes at passage 5 with pLVX-Vecotor or pLVX-ENSMUSG000000648682. qRT-PCR = real-time quantitative polymerase chain reaction. * indicates P < 0.05 and ** indicates P < 0.01 compared with passage 1.