FIGURE 1.

Scutellarin alleviated the TNF-α-induced inflammatory response in HNPCs. (A,B) HNPCs were treated with or without TNF-α (10 ng/ml) in the absence or presence of scutellarin (1 μM) for 24 h. Total RNA from each group was extracted, and real-time PCR was performed to examine the mRNA expression of iNOS and COX-2. (C–E) HNPCs were treated with PBS, TNF-α (10 ng/ml), TNF-α (10 ng/ml) +scutellarin (1 μM) for 48 h. The protein extractions of each group were detected by Western blotting with anti-iNOS and antiCOX-2 antibodies. (F–I) HNPCs were cultured as indicated for 48 h. Immunofluorescence cell staining was performed to detect the expression of iNOS and COX-2 (scale bar: 100 μm). Each experiment was performed three times independently. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).