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. 2022 Aug 11;10:883118. doi: 10.3389/fbioe.2022.883118

FIGURE 3.

FIGURE 3

Scutellarin treatment suppressed TNF-α-mediated apoptosis in HNPCs. (A,B) HNPCs were stimulated with PBS, TNF-α (10 ng/ml), TNF-α (10 ng/ml) +scutellarin (1 μM) for 24 h. Total RNA was extracted from each group, and the mRNA expression of Bcl-2 and Bax was assayed using real-time PCR. (C–F) HNPCs were treated as indicated for 48 h. Total protein was extracted from each group of HNPCs, and the protein expression levels of Bcl-2, Bax, and cleaved caspase-3 were assayed using Western blotting. (G,H) TUNEL staining for each indicated group to examine the apoptosis of HNPCs (scale bar: 100 μm). (I–L) Apoptosis of each indicated group was detected by flow cytometry after treatment as indicated for 48 h. (M,N) Calcein/PI staining was performed to detect the death of HNPCs treated as indicated for 48 h (scale bar: 100 μm). Each experiment was performed three times independently. (*p < 0.05, **p < 0.01, ***p < 0.001).