TABLE 3.

Apparent and the relative sizes of the DNA fragments^a as estimated from their respective electrophoretic mobilities^b

Restriction enzyme	Midpoint positionc	Actual size (bp)	Apparent size (bp)	Relative size (apparent size/actual size)
BamHI	239	258	282	1.09
SspI	216	258	315	1.22
NruI	210	258	318	1.23
PvuII	192	258	322	1.25
EcoRV	186	258	322	1.25
XhoI	172	258	322	1.25
SpeI	166	258	318	1.23
BglII	142	258	302	1.17
MluI	136	258	305	1.18

^aGenerated by restriction digestion of pADH.1 with various enzymes. 

^bElectrophoretic mobilities are given in Fig. 5. 

^cRelative to G (EcoRI) = position 1.