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. 2022 Aug 8;16:100382. doi: 10.1016/j.mtbio.2022.100382

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 4 — In vitro evaluation of osteogenic differentiation of BMSCs in 3D-bioprinted scaffolds. (A) Alkaline phosphatase staining of hydrogel scaffolds induced by osteogenesis for 7 and 14 days. (B) Alizarin red S staining of hydrogel scaffolds induced by osteogenesis for 7 and 14 days. (C) Alkaline phosphatase staining of dissolved hydrogel scaffolds induced by osteogenesis for 14 days under microscopic observation. (D) Alizarin red S staining of dissolved hydrogel scaffolds induced by osteogenesis for 14 days under microscopic observation. (E) Quantitative analysis of alkaline phosphatase activity. (n = 4, each group). (F) Quantitative analysis of mineralized nodules. (n = 4, each group). ∗p < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)