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. 2022 Aug 8;16:100382. doi: 10.1016/j.mtbio.2022.100382

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 5 — In vitro angiogenesis assays. (A) Representative confocal fluorescence microscopy images of mCherry-RAOECs, comparing postseeding method with 3D-bioprinted in situ seeding approach. (B) Assessment of uniformity of mCherry-RAOEC seeding. (C) Confocal fluorescence microscopy images assessing vasculogenic capacity of mCherry-RAOECs after 3, 7, and 12 days of perfusion culture. (D) Quantitative analysis of vascularized area. (E) and (F) Tube formation assay of RAOECs and quantitative analysis of formed meshes. (n = 3, each group). (G) and (H) Migration assay and quantitative analysis of RAOEC migration. (n = 3, each group). ∗p < 0.05.