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. 2022 Aug 8;16:100382. doi: 10.1016/j.mtbio.2022.100382

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Fig. 7 — (A) Volcano map of RAOEC differentially expressed genes in GB-R and GB-3PR groups. (B) GO statistical histogram of differentially expressed genes in GB-R and GB-3PR groups. (C) Q-value enrichment map of GB-3PR in biological process. Enrichment ratio was calculated as (differentially expressed genes in this pathway/all differentially expressed genes)/(genes annotated to this pathway/all annotated genes). In filtering out differentially expressed genes, |log2 fold-change| ≥ 1, p-value < 0.05, and Q-value< 0.05 were used as cut-offs. (D) mRNA levels of angiogenesis-related genes CD31, VEGF, PDGF, and HIF1a, as determined by real-time PCR. (n = 3, each group). (E) Protein levels of angiogenesis-related proteins CD31, VEGF, PDGF, and HIF1a, as measured by western blotting. ∗p < 0.05.