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. 2022 Aug 17;3(3):101620. doi: 10.1016/j.xpro.2022.101620

Figure 2.

Figure 2

Timeline and representative pictures of hiPSC generation

The timeline represents the general procedure to obtain hiPSCs from erythroblasts as well as the timing to obtain these cells.

(A–C) Flow cytometry assessment of erythroblast culture before electroporation showing high purity (more than 98%) in CD71+ cells (A), CD36+ cells (B) with combined analysis of double positive cells (C). Red histograms and plot represent the stained cells while grey histograms and plot represent the unstained control.

(D–G) Evolution of the morphology during cellular reprogramming showing erythroblasts culture (D – day 11), early reprogramming events leading to the generation of hiPSCs (E – day 18), an emerging hiPSC clone (F – day 25) and finally an established hiPSC clone (or colony) (G – day 32). Scale bars: 100 μm.