Table 1.

Summary of QCs to be performed before using hiPSC for downstream experiments

QC	Method	Sample type	Kit	Instructions	Frequency
Negative for bacteria, yeast, fungi infection	Visual inspection of antibiotic-free cultures	Live cell culture	N/A	N/A	Every day
Negative for mycoplasma infection	Enzymatic	Cell culture supernatant	MycoAlertTM Mycoplasma Detection Kit	Link	Every 10 passages
Negative for viral infection (HIV, HBV, HCV)	PCR	On donor somatic cells prior to hiPSC reprogramming	N/A	To be done by a clinical lab at enrollment of the donor	Once
Cellular identity	Short tandem Repeat allele profile	Cells	Promega PowerPlex® 16 System	Link	At cell banking
Genomic stability	G-banding karyotyping	Cells	N/A	N/A	Every 10 passages
Morphology	Visual inspection	Live cell culture	N/A	N/A	Every day
Clearing of episomes	PCR	Extracted DNA	Your favorite PCR kit + primers (see Table 2)	N/A	At cell banking
Pluripotent marker expression	Immunocytochemistry on Oct4, Tra-1-60 and Sox2	Fixed cells	N/A (see key resources table for antibodies)	Standard IF staining procedure (Perriot et al., 2018)	Once
Pluripotency	Embryoid body formation and IF staining for Pax6, AFP and SMA	Fixed cells	N/A (see key resources table for antibodies)	See below	Once