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. 2022 Aug 17;3(3):101620. doi: 10.1016/j.xpro.2022.101620

Table 1.

Summary of QCs to be performed before using hiPSC for downstream experiments

QC Method Sample type Kit Instructions Frequency
Negative for bacteria, yeast, fungi infection Visual inspection of antibiotic-free cultures Live cell culture N/A N/A Every day
Negative for mycoplasma infection Enzymatic Cell culture supernatant MycoAlertTM Mycoplasma Detection Kit Link Every 10 passages
Negative for viral infection (HIV, HBV, HCV) PCR On donor somatic cells prior to hiPSC reprogramming N/A To be done by a clinical lab at enrollment of the donor Once
Cellular identity Short tandem Repeat allele profile Cells Promega PowerPlex® 16 System Link At cell banking
Genomic stability G-banding karyotyping Cells N/A N/A Every 10 passages
Morphology Visual inspection Live cell culture N/A N/A Every day
Clearing of episomes PCR Extracted DNA Your favorite PCR kit + primers (see Table 2) N/A At cell banking
Pluripotent marker expression Immunocytochemistry on Oct4, Tra-1-60 and Sox2 Fixed cells N/A (see key resources table for antibodies) Standard IF staining procedure (Perriot et al., 2018) Once
Pluripotency Embryoid body formation and IF staining for Pax6, AFP and SMA Fixed cells N/A (see key resources table for antibodies) See below Once