Antibodies |
|
CD36-FITC 1 mL (1:50) |
Miltenyi |
130-095-470 |
CD71-PE 1 mL (1:100) |
Miltenyi |
130-091-728 |
CD71 Microbeads 2 mL |
Miltenyi |
130-046-201 |
anti-Sox2 mouse IgG (1:200) |
Abcam |
AB79351 |
anti-Tra-1-60 mouse IgG (1:200) |
Millipore |
MAB4360 |
anti-Oct4 mouse IgG (1:200) |
SCB |
sc-5279 |
anti-SMA mouse IgG (1:200) |
Dako |
M1110851 |
anti-Pax6 rabbit IgG (1:100) |
BioLegend |
PRB-278P |
anti-AFP mouse IgG (1:100) |
Sigma-Aldrich |
A8452 |
|
Biological samples |
|
Human blood |
Male or female donor, aged 2–55 |
N/A |
|
Chemicals, peptides, and recombinant proteins |
|
StemSpan SFEM Medium |
StemCell Technologies |
09605 |
Ficoll-Paque PLUS |
Cytiva |
17144003 |
Human Erythropoietin |
R&D Systems |
287-TC-500 |
Human SCF |
R&D Systems |
255-SC-010 |
Human IGF-1 |
Miltenyi |
130-093-885 |
Human IL-3 |
Miltenyi |
13-093-908 |
Dexamethasone powder |
Sigma-Aldrich |
D4902-25MG |
Cholesterol Lipid Concentrate 250× |
Life Technologies |
12531-018 |
Human CD34+ Nucleofector kit, 25 reactions |
Lonza |
VPA-1003 |
ReproTeSR medium |
StemCell Technologies |
5920 |
Matrigel® hESC-Qualified Matrix, ∗LDEV-Free |
Corning |
354277 |
StemMACS iPS-Brew XF, human |
Miltenyi |
130-104-368 |
StemMACS Y27632 in Solution |
Miltenyi |
130-106-538 |
ReLeSR |
StemCell Technologies |
5873 |
Cryostor CS10 |
StemCell Technologies |
7930 |
Bovine serum albumin (BSA) |
Sigma-Aldrich |
A3912 |
EDTA solution 0.5 M |
Sigma-Aldrich |
03690 |
MycoAlertTM Mycoplasma Detection Kit |
Lonza |
LT07-318 |
PowerPlex® 16 System |
Promega |
DC6531 |
DMEM/F-12 |
Life Technologies |
31331028 |
RPMI 1640 Medium |
Life Technologies |
61870010 |
Dimethyl sulfoxide (DMSO) |
Sigma-Aldrich |
D2650-100ML |
Fetal bovine serum (FBS) |
Sigma-Aldrich |
F7524-500ML |
Accutase |
Life Technologies |
A1110501 |
PBS (1×) without Ca Mg Phenol |
Life Technologies |
14190136 |
Paraformaldéhyde 16% EM Grade |
Electron Microscopy Sciences |
15710 |
Cell lifter |
Corning |
3008-COR |
|
Recombinant DNA |
|
pCXLE-hOCT3/4-shp53-F |
Okita et al. (2011) |
Addgene 27077 |
pCXLE-hSK |
Okita et al. (2011) |
Addgene 27078 |
pCXLE-hUL |
Okita et al. (2011) |
Addgene 27080 |
pCXLE-EGFP |
Okita et al. (2011) |
Addgene 27082 |
|
Oligonucleotides |
|
EBNA1_forward |
Perriot et al. (2018) |
Table 2 |
EBNA1_reverse |
Perriot et al. (2018) |
Table 2 |
β-Actin_forward |
Perriot et al. (2018) |
Table 2 |
β-Actin_reverse |
Perriot et al. (2018) |
Table 2 |
|
Other |
|
Amaxa Nucleofector 2b |
Lonza |
AAB-1001 |