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. 2022 Aug 17;3(3):101620. doi: 10.1016/j.xpro.2022.101620
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

CD36-FITC 1 mL (1:50) Miltenyi 130-095-470
CD71-PE 1 mL (1:100) Miltenyi 130-091-728
CD71 Microbeads 2 mL Miltenyi 130-046-201
anti-Sox2 mouse IgG (1:200) Abcam AB79351
anti-Tra-1-60 mouse IgG (1:200) Millipore MAB4360
anti-Oct4 mouse IgG (1:200) SCB sc-5279
anti-SMA mouse IgG (1:200) Dako M1110851
anti-Pax6 rabbit IgG (1:100) BioLegend PRB-278P
anti-AFP mouse IgG (1:100) Sigma-Aldrich A8452

Biological samples

Human blood Male or female donor, aged 2–55 N/A

Chemicals, peptides, and recombinant proteins

StemSpan SFEM Medium StemCell Technologies 09605
Ficoll-Paque PLUS Cytiva 17144003
Human Erythropoietin R&D Systems 287-TC-500
Human SCF R&D Systems 255-SC-010
Human IGF-1 Miltenyi 130-093-885
Human IL-3 Miltenyi 13-093-908
Dexamethasone powder Sigma-Aldrich D4902-25MG
Cholesterol Lipid Concentrate 250× Life Technologies 12531-018
Human CD34+ Nucleofector kit, 25 reactions Lonza VPA-1003
ReproTeSR medium StemCell Technologies 5920
Matrigel® hESC-Qualified Matrix, ∗LDEV-Free Corning 354277
StemMACS iPS-Brew XF, human Miltenyi 130-104-368
StemMACS Y27632 in Solution Miltenyi 130-106-538
ReLeSR StemCell Technologies 5873
Cryostor CS10 StemCell Technologies 7930
Bovine serum albumin (BSA) Sigma-Aldrich A3912
EDTA solution 0.5 M Sigma-Aldrich 03690
MycoAlertTM Mycoplasma Detection Kit Lonza LT07-318
PowerPlex® 16 System Promega DC6531
DMEM/F-12 Life Technologies 31331028
RPMI 1640 Medium Life Technologies 61870010
Dimethyl sulfoxide (DMSO) Sigma-Aldrich D2650-100ML
Fetal bovine serum (FBS) Sigma-Aldrich F7524-500ML
Accutase Life Technologies A1110501
PBS (1×) without Ca Mg Phenol Life Technologies 14190136
Paraformaldéhyde 16% EM Grade Electron Microscopy Sciences 15710
Cell lifter Corning 3008-COR

Recombinant DNA

pCXLE-hOCT3/4-shp53-F Okita et al. (2011) Addgene 27077
pCXLE-hSK Okita et al. (2011) Addgene 27078
pCXLE-hUL Okita et al. (2011) Addgene 27080
pCXLE-EGFP Okita et al. (2011) Addgene 27082

Oligonucleotides

EBNA1_forward Perriot et al. (2018) Table 2
EBNA1_reverse Perriot et al. (2018) Table 2
β-Actin_forward Perriot et al. (2018) Table 2
β-Actin_reverse Perriot et al. (2018) Table 2

Other

Amaxa Nucleofector 2b Lonza AAB-1001