Figure 2.

FGF21 signaling to GABAergic neurons is not required to promote weight loss in DIO mice. (A–G) 16–18 week old diet-induced obese (DIO) WT and KLB^Vgat−KO mice were administered vehicle or FGF21 (1 mg/kg/day) by osmotic minipump for 2 weeks (n = 6–9/group). (A) Plasma FGF21 levels, (B) daily percent change in body weight, (C) final percent change in body weight, (D) plasma insulin levels, and (E–G) brown adipose tissue mRNA expression of (E) Ucp1, (F) Bmp8b, and (G) Elovl3. Values are mean ± SEM. ∗p < 0.05. Statistical analyses were conducted using two-way ANOVA with Holms-Sidak's multiple comparisons test.