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Overexpression of NarX. (A) SDS-PAGE of membrane fractions prepared from strains lacking the narX overexpression plasmid pET3a-4 (lane 1) or containing pET3a-4 induced with IPTG (lane 2). Equal amounts of total membrane proteins were loaded in lanes 1 and 2. The host strain was BL21(λDE3)/pLysE that was induced with IPTG as indicated in Materials and Methods. (B) Western blot analysis of the corresponding membrane fractions shown in panel A.
