TABLE 1.

Features of the eut locus sequence

Namea	Gene organizationb				Codonsc				Protein			Comment
	Start	Stop	Sense	Overlap	GC	3d.GC	CAI	χ2	Length (aa)	MW	pI
′maeB	−1221	−766	→	−	0.55	0.60	0.292	0.817	>151	>1,610	6.9	One of two malic enzymes
IS200	−721	0	→									IS200 element; copy V
tnpA	−578	−120	→	−	0.46	0.56	0.250	0.572	152	17,958	10.1	IS200 transposase (Tnp)
orf′	1	57	←	−	0.58	0.79	0.192	1.926	19	1,907	7.5	atsB-like fragment
eutS	361	696	→	−	0.51	0.54	0.291	1.128	111	11,673	5.7	Carboxysome structural protein?
eutP	709	1188	→	+	0.52	0.54	0.243	0.645	159	17,726	5.9	Unknown function; ATP binding motif
eutQ	1166	1855	→	+	0.57	0.68	0.398	1.691	229	24,992	4.7	Unknown function
eutT	1852	2655	→	+	0.59	0.68	0.298	1.155	267	30,239	6.4	Cobalamin adenosyl transferase
eutD	2652	3668	→	−	0.62	0.68	0.336	1.373	338	36,266	7.5	Resembles substrate domain of Pta
eutM	3709	3999	→	−	0.60	0.67	0.411	2.002	96	9,843	6.5	Carboxysome structural protein?
eutN	4099	4398	→	−	0.57	0.65	0.315	2.094	99	10,351	5.1	Carboxysome structural protein?
eutE	4410	5813	→	−	0.60	0.69	0.354	1.360	467	49,259	6.9	Aldehyde oxidoreductase
eutJ	5824	6663	→	+	0.61	0.72	0.342	1.105	279	30,018	4.9	Possible chaperonin
eutG	6653	7828	→	−	0.57	0.71	0.320	1.429	391	40,570	7.2	Alcohol dehydrogenase
eutH	7948	9195	→	+	0.57	0.73	0.361	1.489	372	39,053	5.8	Membrane protein
eutA	9192	10595	→	−	0.61	0.71	0.297	0.899	467	49,527	5.0	Possible chaperonin
eutB	10607	11968	→	+	0.57	0.73	0.425	1.265	453	49,449	4.7	ET/NH4d subunit
eutC	11987	12883	→	−	0.61	0.70	0.364	1.475	298	32,137	5.9	ET/NH4 subunit
eutL	12893	13552	→	−	0.60	0.64	0.320	0.841	219	22,696	4.5	Carboxysome structural protein?
eutK	13565	14059	→	−	0.60	0.61	0.289	1.092	164	17,421	7.5	Carboxysome structural protein?
PR	14078		→									Constitutive internal promoter
eutR	14107	15159	→	−	0.54	0.61	0.280	0.815	350	40,055	7.2	AraC family positive regulator
term	15677		→									Probable terminator
orf79	15828	16619	←	−	0.53	0.54	0.297	0.382	263	30,021	4.8	Unknown
term	16673		←									Probable terminator
orf33	16751	17077	←	−	0.43	0.45	0.201	0.788	109	12,020	8.4	Unknown
hemF	17098	17997	←	−	0.57	0.65	0.353	1.213	299	34,430	6.4	Coproporphyrinogen oxidase
	18000	18869	←		0.51	0.54	0.310	0.555	289	31,659	11.0	N-acetylmuramylalanine amidase

^aComponent of the eut operon. The vertical line indicates the elements included in the eut operon. 

^bThe column headed “sense” gives the transcriptional direction, with right-facing arrows indicating counterclockwise on the standard Salmonella map; “overlap” indicates whether or not (+ and −, respectively) the stop codon overlaps the initiation codon of the next gene, implying potential translational coupling or interference (40a). Start and stop codon positions are in nucleotides numbered from the end of IS200. 

^cGC, fractional G+C content of the gene; 3d.GC, G+C content of codon third positions; CAI, codon adaptation index of Sharp and Li (52a); χ², measure of codon usage bias (55a). 

^dET/NH₄, ethanolamine ammonia lyase.