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. 2022 Aug 11;13:959641. doi: 10.3389/fpls.2022.959641

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Copyright © 2022 Wang and Dean.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confirm resistant host-induced gene silencing (HIGS) transgenic rice in T2 generation. (A–F) Punch inoculation of HIGS transgenic rice plants targeting Magnaporthe oryzae gene CRZ1 (A,B), MAGB (C,D), CYP51 (E,F). Wild type Nipponbare (NPB) and non-transgenic segregants derived from corresponding T1 plants were used as negative control. Lesion area was measured and analyzed statistically (B,D,F) using multiple rice lines. The symbol “+” represents positive transgenic plants while “-” represents non-transgenic segregants. Ordinary one-way ANOVA and Turkey’s multiple comparison were used for statistics analysis. ^***P < 0.0002, ^****P < 0.0001.