Figure 1.

Effect of RNA aptamers on porcine VSMC migration

Cells were cultured to confluence followed by 2-day serum starvation then subject to scratch wound injury and treated with PDGF-BB (5 ng/mL) in the absence or presence of aptamers. Aptamers or vehicle were added to cells 30 min before PDGF. Cell images were captured every 2 h and wound confluence was calculated by the IncuCyte S3 system for more than 22 h. Migration was assessed by wound confluence. (A) Aptamer 14 (Apt 14) but not Apt 01 or NSC decreases PDGF-induced VSMC migration. Aptamers was added at 200 nM. n = 8–12. (B) Dose-response of Apt 14 in inhibition of PDGF-mediated cell migration. n = 3–4. (C) Effect of RNA aptamers (200 nM) on cell proliferation to PDGF-BB (2.5 ng/mL). Aptamers were added 30 min before PDGF. Cells were treated with PDGF for 24 h n = 3–4. (D) Representative cell phase images showing the wound confluence at 0 h and 22 h after PDGF exposure. The initial scratch wound and scratch wound area were masked in blue and yellow, respectively. Migration data were analyzed by two-way ANOVA, repeated measures, with Bonferroni post tests. Proliferation data were analyzed by one-way ANOVA with Tukey post hoc test. ^∗p < 0.05, ^∗∗p < 0.01 vs. vehicle, ^#p < 0.05, ^##p < 0.01 vs. PDGF.