Figure 2.

RNA aptamer 14 (Apt 14) inhibits porcine VSMC migration in the presence of contrast agent

Cells were cultured to confluence then subject to scratch wound injury and treated with PDGF-BB (5 ng/mL). Apt 14 was diluted in isotonic contrast agent at a concentration of 200 nM or 500 nM. (A) Wound confluence was measured by the Incucyte S3 system for more than 22 h. (B) Representative cell phase images were shown at 0 h and 22 h after PDGF exposure. The initial scratch wound and scratch wound area were masked in blue and yellow, respectively. Data were analyzed by two-way ANOVA, repeated measures, with Bonferroni post tests. ^∗p < 0.05, ^∗∗p < 0.01 vs. vehicle, ^#p < 0.05, ^##p < 0.01 vs. PDGF. n = 4.