TABLE 3.
Effect of the chrAD50N mutation on the expression of a PhmuO-lac fusion in DH5α
| Plasmids | Hemea | IPTG | LacZ Ub |
|---|---|---|---|
| pWBH20-D50N, pCPO-1, pSHU9 | − | − | 1.1 |
| pWBH20-D50N, pCPO-1, pSHU9 | + | − | 3.6 |
| pWBH20, pCPO-1, pSHU9c | − | − | 8.1 |
| pWBH20, pCPO-1, pSHU9 | + | − | 66.4 |
| pPBH2-D50N, pCPO-1 | − | − | 1.4 |
| pPBH2-D50N, pCPO-1 | − | + | 32.4 |
| pPBH2, pCPO-1 | − | − | 1.5 |
| pPBH2, pCPO-1 | − | + | 61.8 |
a
Hemin was added to cultures (+) at 100 μg/ml.
b
LacZ units (U) were determined by the method of Miller (27). Values are means of three independent experiments, and standard deviations did not vary by greater than 15% from the mean.
c
Plasmid pSHU9 enables DH5α to transport heme.