Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Aug 25;21(12):881–898. doi: 10.1038/s41573-022-00542-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Springer Nature Limited 2022, Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.

PMC Copyright notice

Fig. 5 — a | Example of a reactive probe for activity-based protein profiling (ABPP) designed with a broadly reactive electrophilic warhead linked to an analytical handle. b | Schematic of the competitive isoTOP-ABPP (isotopic tandem orthogonal proteolysis–activity-based protein profiling) methodology. Treatment of cells or lysate with a protein-reactive compound prevents subsequent binding of the pan-reactive probe, and this competitive ligand binding can be detected by tandem mass spectrometry (MS/MS) after an enrichment step, to indirectly identify covalent protein targets for the compound of interest. TEV, tobacco etch virus.