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Cold-protective effect of various isoleucine concentrations. Cells were grown under conditions identical to those for Fig. 1. Different final concentrations of isoleucine, added 2 min after cold shock, were tested for their cold-protective efficiency. ■, 50 μM isoleucine; ●, 20 μM isoleucine; ⧫, 10 μM isoleucine; ▴, 8 μM isoleucine; ▾, 4 μM isoleucine; □, 2 μM isoleucine.
