表 1.
6种有效成分的线性关系、检出限和定量限(n=3)
| Compound | tR/min | Linear range/(μg/L) | Calibration curve | r | LOD/(μg/L) | LOQ/(μg/L) |
|---|---|---|---|---|---|---|
| Shikonin | 6.08±0.01 | 10.00-50.00 | Y=79583X-90506 | 0.9990 | 1.73 | 5.78 |
| Acetylshikonin | 9.76±0.02 | 50.00-250.00 | Y=84361X+139619 | 0.9997 | 0.75 | 4.08 |
| β-Acetoxyisovaleryl akanin | 14.45±0.01 | 40.00-200.00 | Y=71421X-81331 | 0.9999 | 2.24 | 6.38 |
| Isobutyryl shikonin | 18.03±0.03 | 60.00-300.00 | Y=81910X-7028.4 | 0.9985 | 1.58 | 6.55 |
| β,β-Dimethylacryl shikonin | 21.81±0.05 | 50.00-250.00 | Y=109607X-699084 | 0.9990 | 2.63 | 7.67 |
| 2-Methylbutyryl | 24.28±0.05 | 100.00-500.00 | Y=75658X-935990 | 0.9991 | 4.62 | 20.5 |
* Y: peak area of each component; X: mass concentration, μg/L.