表 4.
HPLC分析方法的加样回收率结果(n=6)
| Compound | Background/ (μg/L) |
Added/ (μg/L) |
Found/ (μg/L) |
Recovery/ % |
RSD/ % |
|---|---|---|---|---|---|
| Shikonin | 10.27 | 8.50 | 18.15 | 92.68 | 4.25 |
| 10.27 | 10.00 | 20.13 | 98.58 | ||
| 10.27 | 12.50 | 22.85 | 100.65 | ||
| Acetylshikonin | 86.63 | 70.00 | 152.95 | 94.74 | 5.35 |
| 86.63 | 86.50 | 171.31 | 97.89 | ||
| 86.63 | 104.00 | 195.93 | 105.09 | ||
| β-Acetoxyisovaleryl | 71.42 | 57.50 | 123.43 | 90.45 | 4.19 |
| akanin | 71.42 | 71.50 | 141.70 | 98.29 | |
| 71.42 | 85.50 | 153.08 | 95.50 | ||
| Isobutyryl shikonin | 63.14 | 50.50 | 111.68 | 96.12 | 7.16 |
| 63.14 | 63.00 | 132.08 | 109.42 | ||
| 63.14 | 75.50 | 145.26 | 108.77 | ||
| β,β-Dimethylacryl | 75.81 | 60.50 | 132.57 | 93.81 | 8.35 |
| shikonin | 75.81 | 75.50 | 149.23 | 97.24 | |
| 75.81 | 90.50 | 175.10 | 109.71 | ||
| 2-Methylbutyryl | 148.17 | 118.50 | 251.29 | 87.02 | 4.76 |
| 148.17 | 148.00 | 289.20 | 95.29 | ||
| 148.17 | 178.00 | 314.86 | 93.64 |