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. Author manuscript; available in PMC: 2022 Aug 25.
Published in final edited form as: Immunohorizons. 2021 Jan 21;5(1):33–47. doi: 10.4049/immunohorizons.2000096

FIGURE 4. Th1, Th2, and IL-17 levels in lung tissue.

FIGURE 4.

Protein was extracted from lung tissue, and cytokines were quantified using an MSD U-PLEX Platform. Using the 10-spot MSD U-PLEX Platform, the protein was analyzed for Th1 cytokines (IFN-γ, IL-1β, IL-2, KC, IL-12p70, and TNF-α), Th2 cytokines (IL-4, IL-5, IL-10, and IL-13), and IL-17. From the Th1 cytokines, only IFN-γ, IL-1β, KC, and TNF-α were significantly different in control groups (A–D) but similar after HDME exposure (E–H). Among Th2 cytokines, IL-4, IL-10, and IL-13 cytokines were affected by housing or gender (I–N). IL-17 levels are shown in (O and P). Each dot represents data from one mouse. Ten mice in each group were used.