Table 1. Predicted Aggregation Hotspots, HiSol Scores, and Conserved Residues Corresponding to Residues in XdPH^a.

target residue	HiSol score	conserved residue	appearance rate (%)	position	prediction method
Glu15	–1.103	Ala	9 → 18.4	helix	HiSol
Ile28	2.042	Pro	1.4 → 13.6	coil	HiSol
Val31	2.035	Glu	3.6 → 34.5	coil	HiSol
Val62	2.24	Asp	0 → 29.1	coil	HiSol
Cys76	1.06	Tyr	0 → 54.8	helix	HiSol + α
Leu89	2.021	Arg	5.5 → 25.6	helix	HiSol
Ile108	2.487	Arg	0.2 → 64.2	coil	HiSol
Leu142	2.228	Arg	1.2 → 53.1	coil	HiSol
Cys189	1.132	Asp	0 → 11.5	coil	HiSol
Leu208	1.63	Glu	12.7 → 26.6	helix	HiSol + α
Arg209	–1.355	Leu	24.8 → 18.4	helix	HiSol + α
His218	–1.784	Val	0.5 → 24	helix	HiSol
Arg243	–1.205	Gly	3.8 → 21.2	helix	HiSol
Glu244	–1.077	Ala	14.8→ 19.5	helix	HiSol
Tyr245	–1.181	Val	0 → 26.1	helix	HiSol + α
Phe246	1.051	Tyr	18.9 → 49.9	helix	HiSol
Leu248	1.244	Trp	18.8 → 45.9	helix	HiSol + α
Arg262	–2.271	Val	3.6 → 28.7	helix	HiSol
Ile271	1.682	Tyr	0 → 37.5	helix	HiSol

^aα: α-helix rule; HiSol: hydropathy contradiction rule.